With so many mass spectrometry (MS) systems on the market, it’s easy to feel overwhelmed, especially if you’re new to the field. From single quadrupole systems to triple quadrupole and hybrid analyzers, selecting the right instrument can feel like navigating a maze of acronyms and specifications.
But no worries! In this blog post, we’ll simplify your decision-making process by focusing on the Orbitrap Systems produced by Thermo Fisher Scientific. We’ll explain how Orbitrap works, why it’s trusted in analytical labs worldwide, and introduce three powerful Orbitrap models recently added to our inventory—each offering unique strengths to support your research.
What Is an Orbitrap Mass Spectrometer?
Orbitrap is a high-resolution mass spectrometry platform known for delivering sub-ppm mass accuracy, exceptional sensitivity, and broad dynamic range. Unlike traditional quadrupole or time-of-flight systems, the Orbitrap analyzer measures ions based on the frequency of their oscillations in an electrostatic field, offering unmatched precision. Orbitrap instruments are commonly integrated with liquid chromatography (LC) systems to enhance compound separation and support high-throughput, high-confidence workflows.
How Does the Orbitrap Analyzer Work?
When ions enter the Orbitrap mass analyzer, they are “electrodynamic squeezing” and begin oscillating around a central spindle-like electrode. Each ion’s oscillation frequency is proportional to its mass-to-charge (m/z) ratio. These oscillations generate an image current that is then transformed into mass spectra using a Fourier Transform algorithm.
Why is Orbitrap MS a benefit to your lab?
While Orbitrap technology is considered cutting-edge today, its theoretical origins date as far back as 1923, as outlined in this Analytical Chemistry article. Since then, the Orbitrap MS has continuously evolved and advanced to meet the growing demands of modern laboratory research. It remains a popular choice in the industry, especially in proteomics and structural biology, pharmaceutical quality control, food and environmental safety testing, and clinical analysis.
Here’s why labs around the world rely on Orbitrap technology:
1. Exceptional Mass Accuracy and Resolution
Orbitrap systems consistently deliver sub-ppm mass accuracy and resolving power up to 500,000 FWHM at m/z 200—far exceeding the capabilities of many time-of-flight or quadrupole instruments. This enables:
- Confident identification of unknowns in complex mixtures
- Clear separation of isobaric compounds
- High-fidelity full-scan and MS/MS analysis
For applications such as metabolomics, proteomics, and impurity profiling, this level of precision is essential.
2. Versatile Qualitative and Quantitative Performance
Orbitrap MS platforms support both targeted quantification and untargeted discovery in a single run, making them ideal for multi-purpose workflows. They are equipped to perform:
- Full-scan MS with ultra-high resolution
- MS/MS fragmentation (HCD, CID, ETD, etc.) for structural analysis
- Parallel reaction monitoring (PRM) for high-precision quantitation
This dual capability minimizes sample use, reduces run times, and improves overall productivity.
3. Retrospective Data Analysis
A unique advantage of full-scan HRAM data is the ability to reprocess previously acquired data to investigate new targets—without re-injecting the sample.
Spend wisely with professional support on Refurbished Orbitrap MS
Purchasing a refurbished Orbitrap MS can save you 30% to 70% compared to new systems. At SpectraLab Scientific, each system is professionally refurbished, tested, and validated—backed by expert installation and service support.
We’re excited to introduce three Orbitrap mass spectrometry systems recently added to our inventory. Each model offers distinct advantages based on your lab’s requirements.
Thermo Orbitrap Fusion Lumos Tribrid MS in original crate
This system delivers unmatched performance for complex applications such as low-level post-translational modification (PTM) analysis, isobaric tag quantitation, intact protein characterization, and MSⁿ of small molecules. Featuring advanced ion optics, high-efficiency fragmentation (including ETD HD and optional UVPD), and ultra-high resolution up to 1,000,000 FWHM, it sets a new standard in structural elucidation and quantitative accuracy. Innovations like the Advanced Peak Determination (APD) algorithm ensure smarter, more sensitive data acquisition.
Thermo Orbitrap Fusion Tribrid MS with ETD – SN: FSN1018X
Enhance protein and PTM analysis with Thermo Scientific’s Electron Transfer Dissociation (ETD) option. Unlike CID, ETD preserves labile modifications while fragmenting the peptide backbone, simplifying PTM site identification. It generates complementary c- and z-type ions, boosting sequence coverage and protein identification.
Thermo Orbitrap MS Exploris 120
This system is a Thermo Scientific quadrupole-Orbitrap mass spectrometer that combines proven hardware with next-generation software for fast, reliable performance. Its intuitive interface and ready-to-use acquisition methods make it ideal for walk-up use across applications like food safety, toxicology, environmental testing, metabolomics, and proteomics. Predefined, validated methods ensure consistent results and a quick start for researchers at any level.
| Orbitrap Fusion Lumos Tribrid | Orbitrap Fusion Tribrid with ETD | Orbitrap Exploris 120 | |
| Analyzer Type | Orbitrap + Ion Trap + Quadrupole (Tribrid) | Orbitrap + Ion Trap + Quadrupole (Tribrid) | Orbitrap Only |
| Mass Resolution | Up to 500,000 @ m/z 200 | Up to 450,000 @ m/z 200 | Up to 120,000 @ m/z 200 |
| Fragmentation Modes | CID, HCD, ETD, EThcD, UVPD (optional) | CID, HCD, ETD, EThcD | HCD only |
| ETD Capability | Yes | Yes | No |
| Best For | Advanced proteomics, metabolomics, PTMs | Structural biology, glycomics | Routine quant, small molecule ID |
| Software | Xcalibur, Orbitrap Series Tools | Xcalibur | Xcalibur, TraceFinder |
So, which Orbitrap system is right for you?
Choosing an Orbitrap MS depends on your analytical goals, sample complexity, throughput needs, and budget. Whatever systems or brands you’re looking for, SpectraLab is here to help.
At SpectraLab Scientific, our team offers personalized guidance to help you find the best fit, along with warranty coverage, fast turnaround, full service package, and expert support.
Ready to explore your Orbitrap options?
Contact us today or browse our in stock refurbished Orbitrap mass spectrometers here.
Resources:
- “Orbitrap LC-MS | Thermo Fisher Scientific – US.” 2025. Thermofisher.com. 2025. https://www.thermofisher.com/ca/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms.html?utm_source=chatgpt.com#what-is-orbitrap.
- “Orbitrap Exploris 120 Mass Spectrometer | Thermo Fisher Scientific – US.” Thermofisher.com, 2025, www.thermofisher.com/ca/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers/orbitrap-exploris-120-mass-spectrometers.html. Accessed 25 June 2025.
- Anal. Chem. 2013, 85, 11, 5288–5296
