Includes:

• GE ÄKTA pure 150 M Chromatography System
• Cytiva Fraction Collector F9-C
• Cytiva Sample Pump S9H
• Cytiva Outlet Valve Kit
• PC with UNICORN software data

ÄKTA pure is a flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product. ÄKTA pure chromatography system is a reliable system where hardware and UNICORN software are designed to work together with columns and chromatography resins to meet purification challenges.

The ÄKTA pure 150 was created for optimizing resource utilization and productivity in routine large-scale preparative purification. The system supports a wide range of chromatography techniques and meets automation requirements needed to deliver high purity. You can add on to the system at any time with a wide range of options to further increase its capabilities depending on your purification needs.

ÄKTA pure chromatography system provides you with the following benefits:

• Modular system design with a large range of options to allow flexibility in purification of proteins and peptides
• Intuitive and flexible method creation, system control, and evaluation with UNICORN software
• Practical size, for easy placement on laboratory bench or in cold cabinet
• Reliable system with components and integrated features based on the proven design of ÄKTA protein purification systems
• Predefined method settings for all our laboratory-scale chromatography columns

System Overview

ÄKTA pure chromatography system is a highly versatile, modular system with a number of design features to facilitate reliable purification.

The system consists of the ÄKTA pure instrument and UNICORN software. The system is modular in design with all valves, monitors, and columns mounted on the forward-facing wet side of the system. The design allows easy interaction with the instrument modules. Additional components such as valves, monitors, and sensors from the wide range of optional modules can easily be added to the available positions. Multiple rails for attachment of column holders and equipment are located at the front and on the side of the instrument. A buffer tray on the top of the instrument provides a large storage area for vessels and bottles. The instrument control panel shows the system state and allows the possibility to interact with the run (pause/continue) at the touch of a button.

The system weighs 48 kg in basic configuration and 53 kg when fully equipped. The relatively low weight enables easier placement in the laboratory. The system dimensions allow it to fit conveniently into a standard cold cabinet for work with labile samples.

Regardless of configuration, ÄKTA pure chromatography system always comes with two high-performance system pumps, system pressure monitor for column protection, mixer, injection valve, and UV monitor. ÄKTA pure chromatography system has a wide range of optional modules to allow a large number of possibilities. The system flow path is designed to minimize band-broadening effects, and all wetted materials used in the flow path are biocompatible and resistant to commonly used solvents. The instrument front is designed with empty module positions where optional valves and monitors can be mounted to enable a flexible configuration of the flow path.

UNICORN software allows a fast and easy start to creating methods, controlling runs, and evaluating results. UNICORN software eliminates the need for programming skills as creation of chromatography methods is done by simple drag-and-drop operations. In addition, the software is modular allowing the addition of features such as Column Logbook and Design of Experiments (DoE) functionality for method development. Licensing options for remote access to the system and/or for creating methods or evaluating results give even greater convenience. If preferred, the system can be set up so that it enters “power save mode” after method end, which enables reduction of power consumption by around 80%.

• Wavelength: Multi – choose up to 3 from 190nm to 700nm
• Pressure range: 0 to 5 MPa
• Flow rate: 150 mL/min