The Thermo Scientific Orbitrap Exploris 120 mass spectrometer has demonstrated its unmatched performance in many small molecule application labs around the world. It has redefined productivity in applications such veterinary drug screening, pesticide analysis, drugs of abuse and horse doping analysis.1-6 Now, the full power of Thermo Scientific Orbitrap mass spectrometers can be truly unleashed enabling the analysis of peptides, intact proteins, and oligonucleotides, making this instrument ideal for labs that need to perform every-day targeted and non-targeted work with the absolute confidence that a high resolution, accurate mass instrument can deliver.

The Orbitrap Exploris 120 MS enabled with next-generation instrument control software and modern method editor provides a fast jump start that permits a walk-up system for users that covers typical HRAM applications in food safety, toxicology, environmental, drugs of abuse, metabolomics, medium size proteins and general proteomics applications. The broad selection of predefined acquisition methods tailored to each application and the confidence behind thoroughly tested ready-to-go methods ensures researchers rapid success and consistent results. In addition, the Method Editor and the Tune applications are fully shared across the Thermo Scientific Orbitrap Exploris MS series and with other Thermo Scientific mass spectrometers such as the Thermo Scientific TSQ series triple quadrupole MS and Thermo Scientific Tribrid Orbitrap mass spectrometers reducing the learning curve when transitioning from one analytical platform to another.

The Thermo Scientific Orbitrap Exploris 120 mass spectrometer is a Thermo Scientific quadrupole-Orbitrap mass spectrometer which is based on proven hardware and instrument control software designs of next-generation Thermo Scientific mass spectrometers and incorporates extensive customer and service engineer feedback.1The Orbitrap Exploris 120 mass spectrometer extends the Thermo Scientific Orbitrap Exploris mass spectrometer portfolio, with productivity made easy and rugged for laboratories performing high-throughput targeted screening and quantitation. High-resolution accurate-mass (HRAM) capability provides the fast path to accurate results in a solution with everyday operational usability and consistent performance.

Benefits

• Keep your laboratory running seamlessly with single-click calibration lasting at least five days and maximum mass accuracy using built-in Thermo Scientific EASY-IC ion source internal reference mass
• Focus on your science, not instrument set up with intuitive tuning, drag-and-drop method setup, and predefined experiments driven by next-generation software
• Process more samples with improved scan speed and fast polarity switching
• Improve space utilization and deliver maximum business value with compact footprint when bench-top space is limited

Hardware features
Ion source
Thermo Scientific OptaMax NG ion source

•  Ultimate sensitivity with an efficient, heated electrospray ionization (H-ESI II) probe to deliver maximum performance with minimal optimization
• Additional non-heated low-flow calibrant sprayer for optimal mass and system calibration
• Enhanced system ruggedness and reduced chemical noise with sweep gas
• Flow rates from 1 μL/min to 1,000 μL/min; OPTON-30139 (low-flow needle; 1–10 μL/min), OPTON-30694 (high-flow needle; 5–1,000 μL/min)

Ion optics
The atmospheric pressure ionization (API) interface consists of:

• Round bore transfer tube
• Removable, heated ion transfer tube
• Vent-free maintenance

S-lens
Progressively spaced stacked-ring ion guide (S-Lens) captures and efficiently focuses ions into a tight beam while minimizing in-source dissociation. Large variable spacing between electrodes allows for better pumping efficiency and improved ruggedness.

Advanced active beam guide (AABG)
Axial field and injection filtering reduce noise by preventing neutrals and high-velocity clusters from entering the quadrupole mass filter using a double bent design geometry

Advanced quadrupole technology (AQT)

•  Segmented quadrupole mass filter for precursor ion selection with variable precursor isolation width from 0.4 to 1200 Da
•  MS/MS precursor ion selection with high transmission from m/z 40 to 2,000Ion-routing multipole (IRM)
• Robust ion trapping for MS scans and higher energy collisional dissociation (HCD)
• Selection of absolute collision energy (CE) or normalized collision energy (NCE) for precursor fragmentation
• Nitrogen collision gas

Automatic gain control (AGC)
Reliable AGC measurements for controlled injection of the number of ions is ensured by the novel Independent Charge Detector

Thermo Scientific Orbitrap mass analyzer

• High-field Orbitrap mass analyzer
• Low-noise-detection pre-amplifier
• 4kV central electrode voltage

Vacuum system

•  A compact single turbo pump design provides the adequate vacuum in six stages for the aluminum high-vacuum analyzer chambers
• Advanced vacuum technology reduces pressure in the ultra-high vacuum regions, enhancing transmission of ions to the Orbitrap mass analyzer

Thermo Scientific EASY-IC ion source

• Provides <1 ppm RMS mass accuracy under defined conditions with minimum effort for at least five days
• Generates optional internal reference ions for real-time mass correction of spectra in both positive and negative modes

Optional hardware
APCI probe for OptaMax NG ion source

• Compatible with flow rates from 50 μL/min to 1,000 μL/min without splitting
• The APCI probe can be upgraded to APPI

Specifications

* Under defined conditions

** one cycle consists of acquiring one Full scan in positive mode and one Full scan in negative mode at resolution setting 60,000 one tSIM scan in positive mode and one tSIM scan in negative mode at resolution setting 60,000