Includes:
- 2022 model Wyatt Technologies DAWN Multi-Angle static Light Scattering (MALS) Detector [PN: WD3-03]
- 2022 model Wyatt Technologies Orbit Recycle System [PN: WORB-03]
The DAWN instrument is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. It offers the highest sensitivity, the widest range of molecular weight, size, and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.
MALS is most often used in conjunction with a separation system such as Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS), or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size, and composition, independent of column calibration by reference standards.
The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.
SEC-MALS with a DAWN and Optilab characterizes conformation and branching of synthetic and natural polymers larger than 10 nm in rms radius Rg. The addition of a ViscoStar differential viscometer creates a GPC triple-detection system that can analyze molecular conformation and size below the Rg limit of 10 nm and determine Mark-Houwink Sakurada coefficients.
The combination of a UV/Vis absorbance detector with a DAWN instrument and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a WyattQELS DLS module for size determination, protein conformation may be analyzed.
- Mw from 200 Da to 1000 MDa
- Sensitivity: 10 ng/mL 100 kDa polystyrene in THF, 0.4 µg/mL BSA (66.4 kDa) in PBS
- Rg from 10 to 500 nm, up to 1000 nm with shape-specific models
- Ultra-stable, high-power laser
The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.
In conjunction with a Calypso CG-MALS system, the DAWN characterizes macromolecular interactions, label-free and immobilization-free: affinity and absolute stoichiometry of self- and hetero-association of simple or complex molecular systems. The Calypso system also automates the determination of second virial coefficients, cross virial coefficients, average molar masses and sizes.
Ultrasonic Cleaning Included
Each DAWN instrument includes an embedded COMET ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.
Temperature-Controlled Models
- Ambient model
- Ultra-High-Temperature (UHT) model, from ambient up to +210 °C
- Standard Peltier heated/cooled (H/C) model, temperature control from -15 °C to +150 °C
The Orbit integrates with any Wyatt MALS detector, Optilab RI detector or ViscoStar differential viscometer and even certain third party instruments, to direct the eluent from your flowing system to a waste bottle or back to the solvent reservoir. Program the Orbit to place your system in “recycle” mode at the end of a sample set and your flowing system will be equilibrated and ready upon your return without wasting large quantities of your mobile phase. This automated recycling functionality is critical for chromatography systems whose mobile phases may be costly to purchase/prepare/dispose of and whose column equilibrations could take hours. The unit may be controlled manually via a Wyatt instrument front panel interface or it may be automated within an ASTRA sample set. Also used with Calypso CG-MALS setups to automate switching between wash solvents when cleaning and flushing the system.
Integrates with any flow-based Wyatt instrument to direct the eluent from your flowing system to a waste bottle or back to the solvent reservoir. May be controlled manually via a Wyatt instrument front panel interface, or it may be automated within an ASTRA sample set.
